| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
ip2_1pase
Enzyme Molecule:
IP_1pase | 5.00005 | 2 | 4 | explicit E-S complex | IP2(14)
| IP1(4)
|
| | from Inhorn and Majerus, JBC 1987 Vmax reduced from 5.625 to 2 to maintain levels of IP2(14) |
| 2 |
Enzyme Activity:
ip3_1pase
Enzyme Molecule:
IP_1pase | 20.0021 | 4.125 | 4 | explicit E-S complex | IP3(134)
| IP2(34)
|
| | Ins(134) 1-phosphatase activity of InsP 1-phosphatase from Inhorn and Majerus, JBC 262; 1987 |
| 3 |
Enzyme Activity:
ip3_5pase1
Enzyme Molecule:
IP_5pase1 | 3.00001 | 1.1 | 4 | explicit E-S complex | IP3(145)
| IP2(14)
|
| | Ins(145)P3 5-phosphatase activity of InsP-5-phosphatase from Hansen et al, JBC 262; 1987 |
| 4 |
Enzyme Activity:
ip3_5pase2
Enzyme Molecule:
IP3_5pase2 | 18.0004 | 10.667 | 3.99991 | explicit E-S complex | IP3(145)
| IP2(14)
|
| | from Hansen et al, JBC 262, 1987 activity determined from pH curve. Value scaled 1.5 times to obtain activity at 37C as original experiment was performed at 30C |
| 5 |
Enzyme Activity:
ip4_5pase
Enzyme Molecule:
IP_5pase1 | 0.80003 | 0.09 | 4 | explicit E-S complex | IP4(1345)
| IP3(134)
|
| | Ins(1345)P4 5-phosphatase activity of InsP 5-phosphatase1 from Hansen et al, JBC 262; 1987 vmax for IP4 substrate is approx 0.1 times vmax for IP3 |
