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Accession Type:
Network
Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
Shared_Object_
Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
PKC
PLA2
 Molecule
 Enzyme
 Reaction
PLCbeta
Ras
Gq
MAPK
EGFR
Sos
PLC_g
CaMKII
CaM
PP1
PP2B
PKA
AC
MKP3
PKM

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Enzyme List for pathway PLA2 (Pathway Number 331)

 Molecule Name/
Site Name
Km (uM) kcat (1/s)Ratio
(k2/k3)
Enzyme TypeSubstrate Product
1 Enzyme Activity:
kenz

Enzyme Molecule:
PLA2-Ca*
205.44explicit E-S complexAPC
AA
  10 x raise oct22 12 x oct 24, set k2 = 4 * k3
2 Enzyme Activity:
kenz

Enzyme Molecule:
PIP2-PLA2*
19.999811.044explicit E-S complexAPC
AA
  10 X raise oct 22 12 X further raise oct 24 to allow for correct conc of enzyme
3 Enzyme Activity:
kenz

Enzyme Molecule:
PIP2-Ca-PLA2*
20364explicit E-S complexAPC
AA
  10 x raise oct 22 12 x and rescale for k2 = 4 * k3 convention oct 24 Increase further to get the match to expt, which was spoilt due to large accumulation of PLA2 in the enzyme complexed forms. Lets raise k3, leaving the others at k1 = 1.5e-5 and k2 = 144 since they are large already.
4 Enzyme Activity:
kenz

Enzyme Molecule:
DAG-Ca-PLA2*
19.9996604explicit E-S complexAPC
AA
  10 X raise oct 22 12 X raise oct 24 + conversion to k2 =4 * k3
5 Enzyme Activity:
kenz

Enzyme Molecule:
PLA2*-Ca
20.00021204explicit E-S complexAPC
AA
  This form should be 3 to 6 times as fast as the Ca-only form. I have scaled by 4x which seems to give a 5x rise. 10x raise Oct 22 12 x oct 24, changed k2 = 4 * k3


Pathway Details   Molecule List  Enzyme List   Reaction List  


Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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