NCBS Home page
Accession List
Pathway List
Search
Authorized Users
Help
News archives

Accession Type:
Network
Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
Shared_Object_
Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
PKC
PLA2
PLCbeta
Ras
Gq
MAPK
 Molecule
 Enzyme
EGFR
Sos
PLC_g
CaMKII
CaM
PP1
PP2B
PKA
AC
MKP3
PKM

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By

Enzyme List for pathway MAPK (Pathway Number 335)

 Molecule Name/
Site Name
Km (uM) kcat (1/s)Ratio
(k2/k3)
Enzyme TypeSubstrate Product
1 Enzyme Activity:
MAPKKthr

Enzyme Molecule:
MAPKK*
0.04629630.34explicit E-S complexMAPK-tyr
MAPK*
  Rate consts same as for MAPKKtyr.
2 Enzyme Activity:
MAPKKtyr

Enzyme Molecule:
MAPKK*
0.04629630.34explicit E-S complexMAPK
MAPK-tyr
  The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
3 Enzyme Activity:
Raf*-GTP-Ras.1

Enzyme Molecule:
Raf*-GTP-Ras
0.1590940.34explicit E-S complexMAPKK
MAPKK-ser
  Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
4 Enzyme Activity:
Raf*-GTP-Ras.2

Enzyme Molecule:
Raf*-GTP-Ras
0.1590940.34explicit E-S complexMAPKK-ser
MAPKK*
  Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6


Pathway Details   Molecule List  Enzyme List  Reaction List  


Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.