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Accession Type:
Network
3d_fold_model
Shared_Object_
3d_fold_model
PKC
MAPK
PLA2
Ras
 Molecule
 Enzyme
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Enzyme List for pathway Ras (Pathway Number 58)

 Molecule Name/
Site Name
Km (uM) kcat (1/s)Ratio
(k2/k3)
Enzyme TypeSubstrate Product
1 Enzyme Activity:
GAP-inact-ras

Enzyme Molecule:
GAP
1.010410100explicit E-S complexGTP-Ras
GDP-Ras
  From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting)
2 Enzyme Activity:
GEF*-act-ras

Enzyme Molecule:
GEF*
0.5050510.024explicit E-S complexGDP-Ras
GTP-Ras
  Kinetics same as GEF-bg-act-ras


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