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Accession Type:
Network
Ajay_Bhalla_
2007_ReacDiff2
Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
PKC
MAPK
Ras
CaM
PKM
chain
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[1]
PKC
MAPK
Ras
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[3]
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[2]
PKC
MAPK
Ras
CaM
PKM
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[4]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[5]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[6]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[7]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[8]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[9]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[10]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[11]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[12]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[13]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[14]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[15]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[16]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[17]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[18]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[19]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[20]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[21]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[22]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[23]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[24]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[25]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[26]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[27]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[28]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[29]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[30]
PKC
MAPK
Ras
 Molecule
 Enzyme
 Reaction
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[31]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[32]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[33]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[34]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[35]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[36]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[37]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_Bhalla_
2007_ReacDiff2_
[38]
PKC
MAPK
Ras
CaM
PKM

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Enzyme List for pathway Ras (Pathway Number 867)

 Molecule Name/
Site Name
Km (uM) kcat (1/s)Ratio
(k2/k3)
Enzyme TypeSubstrate Product
1 Enzyme Activity:
basal_GEF_
activity

Enzyme Molecule:
inact-GEF
10.10150.024explicit E-S complexGDP-Ras
GTP-Ras
 
2 Enzyme Activity:
CaM-GEF-act-ras

Enzyme Molecule:
CaM-GEF
0.5050570.24explicit E-S complexGDP-Ras
GTP-Ras
  Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
3 Enzyme Activity:
GAP-inact-ras

Enzyme Molecule:
GAP
1.0104104explicit E-S complexGTP-Ras
GDP-Ras
  From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
4 Enzyme Activity:
GEF*-act-ras

Enzyme Molecule:
GEF*
0.5050570.024explicit E-S complexGDP-Ras
GTP-Ras
  Kinetics same as GEF-bg-act-ras


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Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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