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Accession Type:
Network
CaMKII_model3
Shared_Object_
CaMKII_model3
CaMKII
CaM
PP1
PP2B
PP1_PSD
AC
 Molecule
 Enzyme
 Reaction
PKA

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Reaction List for pathway AC (Pathway Number 269)

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.
  Name KfKbKdtauSubstrateProduct
1 CaM-bind-AC1kf
(uM^-1 s^-1)
1
(s^-1)
Kd(bf) = 0.02(uM)-CaM-Ca4
AC1
AC1-CaM
  Half-max at 20 nM CaM (Tang et al JBC 266:13 8595-8603 1991 kb/kf = 20 nM = 12000 #/cell so kf = kb/12000 = kb * 8.333e-5
2 cAMP_diffusionkf
(s^-1)
5.4
(s^-1)
Not applicable**-cAMP
cAMP_in_dend
  Represents diffusion, from a volume of 9e-20 to 5e-18. Assuming neck dimensions of 0.1 x 0.1 microns, this works out to a diffusion const of about 270 um^2/sec, which is pretty conservative. It is what cAMP does in frog cilia.
3 CaM_bind_PDE1kf
(uM^-1 s^-1)
5
(s^-1)
Kd(bf) = 0.0069(uM)-PDE1
CaM-Ca4
CaM.PDE1
  For olf epi PDE1, affinity is 7 nM. Assume same for brain. Reaction should be pretty fast. Assume kb = 5/sec. Then kf = 5 / (0.007 * 6e5) = 1.2e-3
4 dephosph-AC2kf
(s^-1)
0
(s^-1)
--AC2*
AC2
  Random rate.
5 dephosph-PDEkf
(s^-1)
0
(s^-1)
--cAMP-PDE*
cAMP-PDE
  The rates for this are poorly constrained. In adipocytes (probably a different PDE) the dephosphorylation is complete within 15 min, but there are no intermediate time points so it could be much faster. Identity of phosphatase etc is still unknown.


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