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Accession Type:
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Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
Shared_Object_
Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
PKC
PLA2
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 Reaction
PLCbeta
Ras
Gq
MAPK
EGFR
Sos
PLC_g
CaMKII
CaM
PP1
PP2B
PKA
AC
MKP3
PKM

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Reaction List for pathway PLA2 (Pathway Number 331)

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.
  Name KfKbKdtauSubstrateProduct
1 DAG-Ca-PLA2-actkf
(uM^-1 s^-1)
4
(s^-1)
Kd(bf) = 1333.3467(uM)-DAG
PLA2-Ca*
DAG-Ca-PLA2*
  27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.
2 Degrade-AAkf
(s^-1)
0
(s^-1)
--AA
APC
  I need to check if the AA degradation pathway really leads back to APC. Anyway, it is a convenient buffered pool to dump it back into. For the purposes of the full model we use a rate of degradation of 0.2/sec Raised decay to 0.4 : see PLA35.g notes for Feb17
3 dephosphorylate-
PLA2*
kf
(s^-1)
0
(s^-1)
--PLA2*
PLA2-cytosolic
4 PIP2-Ca-PLA2-actkf
(uM^-1 s^-1)
0.1
(s^-1)
Kd(bf) = 8.3335(uM)-temp-PIP2
PLA2-Ca*
PIP2-Ca-PLA2*
5 PIP2-PLA2-actkf
(uM^-1 s^-1)
0.5
(s^-1)
Kd(bf) = 416.6771(uM)-temp-PIP2
PLA2-cytosolic
PIP2-PLA2*
6 PLA2*-Ca-actkf
(uM^-1 s^-1)
0.1
(s^-1)
Kd(bf) = 0.0167(uM)-PLA2*
Ca
PLA2*-Ca
  To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
7 PLA2-Ca-actkf
(uM^-1 s^-1)
0.1
(s^-1)
Kd(bf) = 0.1(uM)-PLA2-cytosolic
Ca
PLA2-Ca*
  Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267.


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