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Accession Type:
Network
Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
Shared_Object_
Ajay_Bhalla_
2004_PKM_MKP3_
Tuning
PKC
PLA2
 Molecule
 Enzyme
 Reaction
PLCbeta
Ras
Gq
MAPK
EGFR
Sos
PLC_g
CaMKII
CaM
PP1
PP2B
PKA
AC
MKP3
PKM

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Molecule List for pathway PLA2 (Pathway Number 331)

 Name Initial Conc. (uM) Volume (fL) Buffered
1APC301.5Yes
    arachodonylphosphatidylcholine is the favoured substrate from Wijkander and Sundler, JBC 202 pp 873-880, 1991. Their assay used 30 uM substrate, which is what the kinetics in this model are based on. For the later model we should locate a more realistic value for APC.
2DAG-Ca-PLA2*01.5No
3PIP2-Ca-PLA2*01.5No
4PIP2-PLA2*01.5No
5PLA2*01.5No
6PLA2*-Ca01.5No
    Phosphorylated form of PLA2. Still need to hook it up using kinases. PKA: Wightman et al JBC 257 pp6650 1982 PKC: Many refs, eg Gronich et al JBC 263 pp 16645, 1988 but see Lin etal MAPK: Lin et al, Cell 72 pp 269, 1993. Show 3x with MAPK but not PKC alone Do not know if there is a Ca requirement for active phosphorylated state.
7PLA2-Ca*01.5No
8PLA2-cytosolic0.41.5No
    Calculated cytosolic was 20 nm from Wijkander and Sundler However, Leslie and Channon use about 400 nM. Need to confirm, but this is the value I use here. Another recalc of W&S gives 1uM


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