NCBS Home page
Accession List
Pathway List
Search
Authorized Users
Help
News archives

Accession Type:
Network
Ajay_bhalla_
2007_ReacDiff1_
1e-12
Shared_Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12
PKC
MAPK
Ras
CaM
PKM
chain
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[1]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[2]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[3]
PKC
MAPK
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[4]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[5]
PKC
MAPK
Ras
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[6]
CaM
PKM
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[7]
PKC
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[8]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[9]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[10]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[11]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[12]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[13]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[14]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[15]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[16]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[17]
PKC
MAPK
Ras
CaM
 Molecule
 Reaction
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[18]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[19]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[20]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[21]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[22]
PKC
MAPK
Ras
CaM
PKM
Shared Object_
Ajay_bhalla_
2007_ReacDiff1_
1e-12_[23]
PKC
MAPK
Ras
CaM
PKM

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By

Reaction List for pathway CaM (Pathway Number 488)

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.
  Name KfKbKdtauSubstrateProduct
1 CaM-bind-Cakf
(uM^-1 s^-1)
8.4853
(s^-1)
Kd(bf) = 1(uM)-CaM
Ca
CaM-Ca
  Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
2 CaM-Ca-bind-Cakf
(uM^-1 s^-1)
8.4853
(s^-1)
Kd(bf) = 1(uM)-CaM-Ca
Ca
CaM-Ca2
  Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853
3 CaM-Ca2-bind-Cakf
(uM^-1 s^-1)
10
(s^-1)
Kd(bf) = 2.7778(uM)-CaM-Ca2
Ca
CaM-Ca3
  K3 = 21.5, K4 = 2.8. Assuming that the K4 step happens first, we get kb/kf = 2.8 uM = 1.68e6 so kf =6e-6 assuming kb = 10
4 CaM-Ca3-bind-Cakf
(uM^-1 s^-1)
10
(s^-1)
Kd(bf) = 21.5051(uM)-CaM-Ca3
Ca
CaM-Ca4
  Use K3 = 21.5 uM here from Stemmer and Klee table 3. kb/kf =21.5 * 6e5 so kf = 7.75e-7, kb = 10


Pathway Details   Molecule List  Enzyme List  Reaction List  


Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.