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Molecule Parameter List for NOS*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

NOS* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	3	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
NOS_Phosph_ regulation	20	Pathway	NOS
This model features the phosphorylation of rat brain neuronal NOS expressed in E. coli or Sf9 cells, which leads to a decrease in Vmax of the phosphorylated enzyme, with little change of both the Km for L-arginine and Kact for CaM. This is based on Hayashi Y. et al. J Biol Chem. (1999) 274(29):20597-602. They report of phosphorylatin being carried out by CaM kinases I alpha, II alpha and IV. The rates used have been obtained from their paper and from other reported experimental data.

NOS* acting as a Molecule in  NOS_Phosph_regulation Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
NOS*	NOS_Phosph_ regulation Accession No. : 20	NOS Pathway No. : 90	0	0.0016667	No
Phosporylated NOS, by CaM kinases, with lowering of Vmax, but little change in Km for Arg and Kact for CaM. (Hayashi et al., JBC, 1999, 274(29):20597-20602)

NOS* acting as a Product of an Enzyme in  NOS_Phosph_regulation Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	CaMKIV  / kenz	NOS_Phosph_ regulation Accession No. : 20	NOS Pathway No. : 90	5	18	4	explicit E-S complex	Substrate nNOS Product NOS*
	Hayashi et al., 1999, JBC,274(29):20597-20602. and other reported data from different sources.
2	CaMKIIalpha  / kenz	NOS_Phosph_ regulation Accession No. : 20	NOS Pathway No. : 90	5	28.5	4	explicit E-S complex	Substrate nNOS Product NOS*
	Hayashi et al., 1999, JBC,274(29):20597-20602. and from various other literature datas.
3	CaMKIalpha  / kenz	NOS_Phosph_ regulation Accession No. : 20	NOS Pathway No. : 90	5	17	4	explicit E-S complex	Substrate nNOS Product NOS*
	enzyme parameters used from different literature. Hayashi et al., 1999, JBC,274(29):20597-20602.

NOS* acting as a Substrate in a reaction in  NOS_Phosph_regulation Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
dephosporyl	NOS_Phosph_ regulation Accession No. : 20	NOS Pathway No. : 90	13.9 (s^-1)	0 (s^-1)	-	-	Substrate NOS* Product nNOS
kf -13.9 These rates used to keep the basal level of nNOS at reasonable experimental levels.