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Molecule Parameter List for AC2* | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Ajay_Bhalla_
2004_PKM_Tuning | 76 | Network |
PKC, Shared_Object_Ajay_Bhalla_2004_PKM_tuning, PLA2,
PLCbeta, Gq, MAPK,
Ras, EGFR, Sos,
PLC_g, CaMKII, CaM,
PP1, PP2B, PKA,
AC, PKM | | This model is taken from the Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the reference feedforward model from Figure 8a. |
AC2* acting as a Molecule in Ajay_Bhalla_2004_PKM_Tuning Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | AC2* | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | AC
Pathway No. : 327 | 0 | 1.5 | No | | This version is activated by Gs and by a betagamma and phosphorylation. |
AC2* acting as an Enzyme in Ajay_Bhalla_2004_PKM_Tuning Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | AC2* /
kenz
| Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | AC
Pathway No. : 327 | 20.1153 | 7 | 4 | explicit E-S complex | Substrate
ATP
Product
cAMP
| | Reduced Km to match expt data for basal activation of AC2 by PKC. Now k1 = 2.9e-6, k2 = 72, k3 = 18 |
AC2* acting as a Product of an Enzyme in Ajay_Bhalla_2004_PKM_Tuning Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | PKC-active /
phosph-AC2 | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | Shared_Object_
Ajay_Bhalla_
2004_PKM_tuning
Pathway No. : 312 | 33.3332 | 4 | 4 | explicit E-S complex | Substrate
AC2
Product
AC2*
| | Phorbol esters have little effect on AC1 or on the Gs-stimulation of AC2. So in this model we are only dealing with the increase in basal activation of AC2 induced by PKC k1 = 1.66e-6 k2 = 16 k3 =4 |
AC2* acting as a Substrate in a reaction in Ajay_Bhalla_2004_PKM_Tuning Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | dephosph-AC2 | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | AC
Pathway No. : 327 | 0.1
(s^-1) | 0
(s^-1) | - | - | Substrate
AC2*
Product
AC2
| | | Random rate. | | 2 | Gs-bind-AC2* | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | AC
Pathway No. : 327 | 833.283
(uM^-1 s^-1) | 1
(s^-1) | Kd(bf) = 0.0012(uM) | - | Substrate
AC2*
Gs-alpha
Product
AC2*-Gs
| | | kb/kf = 1.2 nM so kf = kb/720 = 1.3888 * kb. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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