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Accession information for cGMP_regulation (Accession Number 34)

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Accession Basic Parameters

Name cGMP_regulation
Accession Type Network
Transcriber Sudhir Sivakumaran, NCBS
Developer Sudhir Sivakumaran, NCBS
Entry Date (YYYY-MM-DD) 2002-08-27
Species Bovine and Sprague-Dawley rats; mammalian
Tissue Pulmonary, cardiac and expressed in Baculovirus infected Sf21(Spodoptera frugiperda) insect cells
Cell Compartment Cytosol
Source In-house
Methodology Quantitative match to experiments, Qualitative
Model Implementation Exact GENESIS implementation
Model Validation Replicates orginal data, Approximates original data
Notes Though Corbin JD. et al. Eur J Biochem. (2000) 267(9):2760-7 has been mentioned in the citation, this model has been made with inputs from different literature sources, each of which has been mentioned in the notes sections. This model features hydrolysis of cGMP by bovine PDE, phosphorylation of PDE by bovine lung PKG, and activation of bovine lung PKG by cGMP binding. These mechanisms are known to be involved in cGMP level regulation. Rates have been used from different sources and the model has been tested based on Corbin JD. et al., since their work involved measuring the PDE phosphorylation and PDE activity.
On replicating Figures 2, 3 and 4 from their paper, there is approx 30% difference in results but the qualitative shape of the curves is very similar. This might be due to the fact that the Vmax values were used from different literature sources. This might lead to the discrepancy in the numbers in this model. The values shown in this model are near estimated physiological levels.In order to replicate the Figures more closely, we have run additional simulations with concentration terms changed so as to replicate the experimental conditions exactly.

Conversion formats

Native Format (GENESIS format)    acc34.g   
GENESIS Format (Annotated version)    Anno_acc34.g   

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