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Result: 1 - 9 of 9 rows are displayed

Enzyme List for pathway AC (Pathway Number 221) in Accession MAPK_network_2003 (Accession Number 50)

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  Enzyme Molecule /

 Enzyme Activity
Pathway Name / 
Pathway No.
Km
(uM)
kcat
(s^-1)
Ratio
(k2/k3)
Enzyme TypeReagents
1 AC1-CaM /
kenz
AC

Pathway No. 221
20184Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate: 
ATP
Product : 
cAMP
2 AC2* /
kenz
AC

Pathway No. 221
20.114974Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate: 
ATP
Product : 
cAMP
  Reduced Km to match expt data for basal activation of AC2 by PKC. Now k1 = 2.9e-6, k2 = 72, k3 = 18
3 AC2-Gs /
kenz
AC

Pathway No. 221
20184Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate: 
ATP
Product : 
cAMP
4 AC1-Gs /
kenz
AC

Pathway No. 221
20184Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate: 
ATP
Product : 
cAMP
5 AC2*-Gs /
kenz
AC

Pathway No. 221
60544Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate: 
ATP
Product : 
cAMP
6 cAMP-PDE /
PDE
AC

Pathway No. 221
19.8413104explicit E-S complexSubstrate: 
cAMP
Product : 
AMP
  Best rates are from Conti et al Biochem 34 7979-7987 1995. Though these are for the Sertoli cell form, it looks like they carry nicely into alternatively spliced brain form. See Sette et al JBC 269:28 18271-18274 Km ~2 uM, Vmax est ~ 10 umol/min/mg for pure form. Brain protein is 93 kD but this was 67. So k3 ~10, k2 ~40, k1 ~4.2e-6
7 cAMP-PDE* /
PDE*
AC

Pathway No. 221
19.8413204explicit E-S complexSubstrate: 
cAMP
Product : 
AMP
  This form has about twice the activity of the unphosphorylated form. See Sette et al JBC 269:28 18271-18274 1994. We'll ignore cGMP effects for now.
8 PDE1 /
PDE1
AC

Pathway No. 221
39.71.6674.0012explicit E-S complexSubstrate: 
cAMP
Product : 
AMP
  Rate is 1/6 of the CaM stim form. We'll just reduce all lf k1, k2, k3 so that the Vmax goes down 1/6.
9 CaM.PDE1 /
CaM.PDE1
AC

Pathway No. 221
39.6825104explicit E-S complexSubstrate: 
cAMP
Product : 
AMP
  Max activity ~10umol/min/mg in presence of lots of CaM. Affinity is low, 40 uM. k3 = 10, k2 = 40, k1 = (50/40) / 6e5.

 
Result: 1 - 9 of 9 rows are displayed



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