| |
Enzyme Molecule /
Enzyme Activity | Pathway Name /
Pathway No. | Km
(uM) | kcat
(s^-1) | Ratio
(k2/k3) | Enzyme Type | Reagents |
| 1 | PLA2-Ca* /
kenz | PLA2
Pathway No. 314 | 20 | 5.4 | 4 | explicit E-S complex | Substrate:
APC
Product :
AA
|
| | 10 x raise oct22 12 x oct 24, set k2 = 4 * k3 |
| 2 | PIP2-PLA2* /
kenz | PLA2
Pathway No. 314 | 19.9998 | 11.04 | 4 | explicit E-S complex | Substrate:
APC
Product :
AA
|
| | 10 X raise oct 22 12 X further raise oct 24 to allow for correct conc of enzyme |
| 3 | PIP2-Ca-PLA2* /
kenz | PLA2
Pathway No. 314 | 20 | 36 | 4 | explicit E-S complex | Substrate:
APC
Product :
AA
|
| | 10 x raise oct 22 12 x and rescale for k2 = 4 * k3 convention oct 24 Increase further to get the match to expt, which was spoilt due to large accumulation of PLA2 in the enzyme complexed forms. Lets raise k3, leaving the others at k1 = 1.5e-5 and k2 = 144 since they are large already. |
| 4 | DAG-Ca-PLA2* /
kenz | PLA2
Pathway No. 314 | 19.9996 | 60 | 4 | explicit E-S complex | Substrate:
APC
Product :
AA
|
| | 10 X raise oct 22 12 X raise oct 24 + conversion to k2 =4 * k3 |
| 5 | PLA2*-Ca /
kenz | PLA2
Pathway No. 314 | 20.0002 | 120 | 4 | explicit E-S complex | Substrate:
APC
Product :
AA
|
| | This form should be 3 to 6 times as fast as the Ca-only form. I have scaled by 4x which seems to give a 5x rise. 10x raise Oct 22 12 x oct 24, changed k2 = 4 * k3 |
and 
color.
arrow indicates that ordering is done according to ascending or descending order.