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Enzyme List for pathway PLCbeta (Pathway Number 332) in Accession Ajay_Bhalla_2004_PKM_MKP3_Tuning (Accession Number 77)

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  Enzyme Molecule /

 Enzyme Activity
Pathway Name / 
Pathway No.
Km
(uM)
kcat
(s^-1)
Ratio
(k2/k3)
Enzyme TypeReagents
1 PLC-Ca /
PLC-Ca
PLCbeta

Pathway No. 332
19.8413104explicit E-S complexSubstrate: 
PIP2
Product : 
DAG
IP3
  From Sternweis et al Phil Trans R Soc Lond 1992, also matched by Homma et al. k1 = 1.5e-5, now 4.2e-6 k2 = 70/sec; now 40/sec k3 = 17.5/sec; now 10/sec Note that the wording in Sternweis et al is ambiguous re the Km.
2 PLC-Ca-Gq /
PLCb-Ca-Gq
PLCbeta

Pathway No. 332
5.00003484explicit E-S complexSubstrate: 
PIP2
Product : 
DAG
IP3
  From Sternweis et al, Phil Trans R Soc Lond 1992, and the values from other refs eg Homma et al JBC 263(14) pp6592 1988 match. k1 = 5e-5/sec k2 = 240/sec; now 120/sec k3 = 60/sec; now 30/sec Note that the wording in Sternweis et al is ambiguous wr. to the Km for Gq vs non-Gq states of PLC. K1 is still a bit too low. Raise to 7e-5 9 Jun 1996: k1 was 0.0002, changed to 5e-5

 
Result: 1 - 2 of 2 rows are displayed



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