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Enzyme List for pathway PLC_g (Pathway Number 338) in Accession Ajay_Bhalla_2004_PKM_MKP3_Tuning (Accession Number 77)

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	Enzyme Molecule / Enzyme Activity	Pathway Name / Pathway No.	Km (uM)	kcat (s^-1)	Ratio (k2/k3)	Enzyme Type	Reagents
1	Ca.PLC_g / PIP2_hydrolysis	PLC_g Pathway No. 338	97.2222	14	4	explicit E-S complex	Substrate: PIP2 Product : DAG IP3
	Mainly Homma et al JBC 263:14 1988 pp 6592, but these parms are the Ca-stimulated form. It is not clear whether the enzyme is activated by tyrosine phosphorylation at this point or not. Wahl et al JBC 267:15 10447-10456 1992 say that the Ca_stim and phosph form has 7X higher affinity for substrate than control. This is close to Wahl's figure 7, which I am using as reference.
2	Ca.PLC_g* / PIP2_hydrolysis	PLC_g Pathway No. 338	19.7917	57	4	explicit E-S complex	Substrate: PIP2 Product : DAG IP3
	Mainly Homma et al JBC 263:14 1988 pp 6592, but these parms are the Ca-stimulated form. It is not clear whether the enzyme is activated by tyrosine phosphorylation at this point or not. Wahl et al JBC 267:15 10447-10456 1992 say that this has 7X higher affinity for substrate than control.

Database compilation and code copyright (C) 2005, Upinder S. Bhalla and NCBS/TIFR
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