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Enzyme Molecule /
Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents |
1 | Ca.PLC_g / PIP2_hydrolysis | PLC_g
Pathway No. 338 | 97.2222 | 14 | 4 | explicit E-S complex | Substrate: PIP2 Product : DAG IP3
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| Mainly Homma et al JBC 263:14 1988 pp 6592, but these parms are the Ca-stimulated form. It is not clear whether the enzyme is activated by tyrosine phosphorylation at this point or not. Wahl et al JBC 267:15 10447-10456 1992 say that the Ca_stim and phosph form has 7X higher affinity for substrate than control. This is close to Wahl's figure 7, which I am using as reference. |
2 | Ca.PLC_g* / PIP2_hydrolysis | PLC_g
Pathway No. 338 | 19.7917 | 57 | 4 | explicit E-S complex | Substrate: PIP2 Product : DAG IP3
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| Mainly Homma et al JBC 263:14 1988 pp 6592, but these parms are the Ca-stimulated form. It is not clear whether the enzyme is activated by tyrosine phosphorylation at this point or not. Wahl et al JBC 267:15 10447-10456 1992 say that this has 7X higher affinity for substrate than control. |