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Result: 1 - 4 of 4 rows are displayed

Enzyme List for pathway Ras (Pathway Number 397) in Accession Ajay_bhalla_2007_ReacDiff1_1e-12 (Accession Number 81)

Entries are grouped according to Pathway Number and they are alternately color coded using  and   color.
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  Enzyme Molecule /

 Enzyme Activity
Pathway Name / 
Pathway No.
Km
(uM)
kcat
(s^-1)
Ratio
(k2/k3)
Enzyme TypeReagents
1 inact-GEF /
  • basal_GEF_
    activity
  • Ras

    Pathway No. 397
    10.10150.024explicit E-S complexSubstrate: 
    GDP-Ras
    Product : 
    GTP-Ras
     
    2 GEF* /
    GEF*-act-ras
    Ras

    Pathway No. 397
    0.5050570.024explicit E-S complexSubstrate: 
    GDP-Ras
    Product : 
    GTP-Ras
      Kinetics same as GEF-bg-act-ras
    3 GAP /
    GAP-inact-ras
    Ras

    Pathway No. 397
    1.0104104explicit E-S complexSubstrate: 
    GTP-Ras
    Product : 
    GDP-Ras
      From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
    4 CaM-GEF /
    CaM-GEF-act-ras
    Ras

    Pathway No. 397
    0.5050570.24explicit E-S complexSubstrate: 
    GDP-Ras
    Product : 
    GTP-Ras
      Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.

     
    Result: 1 - 4 of 4 rows are displayed



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