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Enzyme List for pathway PLCbeta (Pathway Number 73) in Accession Synaptic_Network (Accession Number 16)

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  Enzyme Molecule /

 Enzyme Activity
Pathway Name / 
Pathway No.
Km
(uM)
kcat
(s^-1)
Ratio
(k2/k3)
Enzyme TypeReagents
1 PLC-Ca /
PLC-Ca
PLCbeta

Pathway No. 73
19.8413104explicit E-S complexSubstrate: 
PIP2
Product : 
DAG
IP3
  From Sternweis et al Phil Trans R Soc Lond 1992, also matched by Homma et al. Km of 20 is higher than for the Gq bound form, but Vmax is about 1/3 of the Gq form.
2 PLC-Ca-Gq /
PLCb-Ca-Gq
PLCbeta

Pathway No. 73
5484explicit E-S complexSubstrate: 
PIP2
Product : 
DAG
IP3
  From Sternweis et al, Phil Trans R Soc Lond 1992, and the values from other refs eg Homma et al JBC 263(14) pp6592 1988 match. In this model I have rather low values for PIP2. The Km values are low to match. Sternweis mentions a 5 uM Km which is what I use here, but the Homma paper suggests about 20x higher Km, which would also fit with 20x higher PIP2. So that parameter, though it is off, cancels out and the overall rate would be the same. Vmax is about 23 umol/min/mg at high Ca from Sternweis or about 60/sec. This model value is a little lower than that.

 
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