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Enzyme Molecule /
Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents |
1 | Ca.PLC_g / PIP2_hydrolysis | PLC_g
Pathway No. 79 | 97.2222 | 14 | 4 | Classical Michaelis-Menten V = Etot.S.Kcat/Km+S | Substrate: PIP2 Product : DAG IP3
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| Wahl et al JBC 267(15) 10447-10456 1992. Homma et al JBC 263:14 1988 pp 6592. These parms are the Ca-stimulated form. This is close to Wahl's figure 7, which I am using as reference. Also see Nakanishi et al Biochem J 256 453-459 1998, Nishibe et al Science 250 :1253-1256 This model uses a rather low PIP2 of 10 uM. |
2 | Ca.PLC_g* / PIP2_hydrolysis | PLC_g
Pathway No. 79 | 19.7917 | 57 | 4 | Classical Michaelis-Menten V = Etot.S.Kcat/Km+S | Substrate: PIP2 Product : DAG IP3
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| Mainly Homma et al JBC 263:14 1988 pp 6592. These parms are the Ca-stimulated form. Wahl et al JBC 267:15 10447-10456 1992 say that the tyrosine phosphorylated form has 7X higher affinity for substrate than control. The PIP2 levels in this model are rather low, at 10 uM. |