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Molecule List for pathway PLA2 (Pathway Number 183) in Accession MAPK-bistability-fig1c (Accession Number 35)

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The entries are grouped according to Pathway Number and are alternately color coded using  and  color.

BufferedSum Total Of
1 APCNetwork301000Yes
    arachodonylphosphatidylcholine is the favoured substrate from Wijkander and Sundler, JBC 202 pp 873-880, 1991. Their assay used 30 uM substrate, which is what the kinetics in this model are based on. For the later model we should locate a more realistic value for APC. For now it is treated as a buffered metabolite.
2 DAG-Ca-PLA2*Network01000No
3 PIP2-Ca-PLA2*Network01000No
4 PIP2-PLA2*Network01000No
5 PLA2*Network01000No
    Phosphorylated PLA2. The site differs from the site phosphorylated by PKC. See Nemenoff et al 1993 JBC 268(3):1960-1964
6 PLA2*-CaNetwork01000No
    Phosphorylated and active form of PLA2. Several kinases act on it: PKA: Wightman et al JBC 257 pp6650 1982 PKC: Many refs, eg Gronich et al JBC 263 pp 16645, 1988 but see Lin etal MAPK: Lin et al, Cell 72 pp 269, 1993. Show 3x with MAPK but not PKC alone The Nemenoff assays are conducted in rather high Ca so I have assumed a Ca binding step.
7 PLA2-Ca*Network01000No
    The generic Ca-activated form ofPLA2. Leslie and Channon 1990 BBA 1045:261.
8 PLA2-cytosolicNetwork0.41000No
    cPLA2 IV form has mol wt of 85 Kd. Glaser et al 1993 TIPS 14:92-98. Calculated cytosolic concentration is ~300 nM from Wijkander and Sundler 1991 Eur J Biochem 202:873 Leslie and Channon 1990 BBA 1045:261 use about 400 nM. Decent match. Use 400 nM.

Result: 1 - 8 of 8 rows are displayed

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