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Result: 1 - 10 of 10 rows are displayed

Reaction List for pathway PKC (Pathway Number 207) in Accession MAPK_network_2003 (Accession Number 50)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name
Pathway Name / 
Pathway No.
KfKbKdtauReagents
1 PKC-act-by-CaPKC

Pathway No. 207
0.6
(uM^-1 s^-1)
0.5
(s^-1)
Kd(bf) = 0.8333(uM)-  Substrate:
 PKC-cytosolic
 Ca

 Products:
 PKC-Ca
  Need est of rate of assoc of Ca and PKC. Assume it is fast The original parameter-searched kf of 439.4 has been scaled by 1/6e8 to account for change of units to n. Kf now 8.16e-7, kb=.6085 Raised kf to 1e-6 to match Ca curve, kb to .5
2 PKC-act-by-DAGPKC

Pathway No. 207
0.008
(uM^-1 s^-1)
8.6348
(s^-1)
Kd(bf) = 1079.377(uM)-  Substrate:
 DAG
 PKC-Ca

 Products:
 PKC-Ca-DAG
  Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
3 PKC-Ca-to-membPKC

Pathway No. 207
1.2705
(s^-1)
3.5026
(s^-1)
Keq = 2.7569(uM)0.21sec  Substrate:
 PKC-Ca

 Products:
 PKC-Ca-memb*
4 PKC-DAG-to-membPKC

Pathway No. 207
1
(s^-1)
0.1
(s^-1)
Keq = 0.1(uM)0.909sec  Substrate:
 PKC-Ca-DAG

 Products:
 PKC-DAG-memb*
  Raise kb from .087 to 0.1 to match data from Shinomura et al. Lower kf from 1.155 to 1.0 to match data from Shinomura et al.
5 
  • PKC-act-by-Ca-AA
  • PKC

    Pathway No. 207
    0.0012
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 83.3333(uM)-  Substrate:
     PKC-Ca
     AA

     Products:
     PKC-Ca-AA*
      Schaechter and Benowitz We have to increase Kf for conc scaling Changed kf to 2e-9 on Sept 19, 94. This gives better match.
    6 
  • PKC-act-by-DAG-A
    A
  • PKC

    Pathway No. 207
    2
    (s^-1)
    0.2
    (s^-1)
    Keq = 0.1(uM)0.455sec  Substrate:
     PKC-DAG-AA

     Products:
     PKC-DAG-AA*
      Assume slowish too. Schaechter and Benowitz
    7 PKC-basal-actPKC

    Pathway No. 207
    1
    (s^-1)
    50
    (s^-1)
    Keq = 50(uM)0.02sec  Substrate:
     PKC-cytosolic

     Products:
     PKC-basal*
      Initial basal levels were set by kf = 1, kb = 20. In model, though, the basal levels of PKC activity are higher.
    8 PKC-act-by-AAPKC

    Pathway No. 207
    0.0001
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 833.3333(uM)-  Substrate:
     AA
     PKC-cytosolic

     Products:
     PKC-AA*
      Raise kf from 1.667e-10 to 2e-10 to get better match to data.
    9 PKC-n-DAGPKC

    Pathway No. 207
    0.0006
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 166.6667(uM)-  Substrate:
     PKC-cytosolic
     DAG

     Products:
     PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    10 PKC-n-DAG-AAPKC

    Pathway No. 207
    0.018
    (uM^-1 s^-1)
    2
    (s^-1)
    Kd(bf) = 111.1111(uM)-  Substrate:
     PKC-DAG
     AA

     Products:
     PKC-DAG-AA
      Reduced kf to 0.66X to match Shinomura et al data. Initial: kf = 3.3333e-9 New: 2e-9 Newer: 2e-8 kb was 0.2 now 2.

     
    Result: 1 - 10 of 10 rows are displayed



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