NCBS Home page
Accession List
Pathway List
Search
Authorized Users
Help
News archives

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By


 
Result: 1 - 7 of 7 rows are displayed

Reaction List for pathway AC (Pathway Number 221) in Accession MAPK_network_2003 (Accession Number 50)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name
Pathway Name / 
Pathway No.
KfKbKdtauReagents
1 CaM-bind-AC1AC

Pathway No. 221
49.9998
(uM^-1 s^-1)
1
(s^-1)
Kd(bf) = 0.02(uM)-  Substrate:
 CaM-Ca4
 AC1

 Products:
 AC1-CaM
  Half-max at 20 nM CaM (Tang et al JBC 266:13 8595-8603 1991 kb/kf = 20 nM = 12000 #/cell so kf = kb/12000 = kb * 8.333e-5
2 dephosph-AC2AC

Pathway No. 221
0.1
(s^-1)
0
(s^-1)
--  Substrate:
 AC2*

 Products:
 AC2
  Random rate.
3 Gs-bind-AC2AC

Pathway No. 221
499.998
(uM^-1 s^-1)
1
(s^-1)
Kd(bf) = 0.002(uM)-  Substrate:
 AC2
 Gs-alpha

 Products:
 AC2-Gs
  Half-max at around 3nM = kb/kf from fig 5 in Feinstein et al PNAS USA 88 10173-10177 1991 kf = kb/1800 = 5.56e-4 kb Ofer's thesis data indicates it is more like 2 nM. kf = kb/1200 = 8.33e-4
4 Gs-bind-AC1AC

Pathway No. 221
126
(uM^-1 s^-1)
1
(s^-1)
Kd(bf) = 0.0079(uM)-  Substrate:
 Gs-alpha
 AC1

 Products:
 AC1-Gs
  Half-max 8nM from Tang et al JBC266:13 8595-8603 kb/kf = 8 nM = 4800#/cell so kf = kb * 2.08e-4
5 Gs-bind-AC2*AC

Pathway No. 221
833.28
(uM^-1 s^-1)
1
(s^-1)
Kd(bf) = 0.0012(uM)-  Substrate:
 Gs-alpha
 AC2*

 Products:
 AC2*-Gs
  kb/kf = 1.2 nM so kf = kb/720 = 1.3888 * kb.
6 dephosph-PDEAC

Pathway No. 221
0.1
(s^-1)
0
(s^-1)
--  Substrate:
 cAMP-PDE*

 Products:
 cAMP-PDE
  The rates for this are poorly constrained. In adipocytes (probably a different PDE) the dephosphorylation is complete within 15 min, but there are no intermediate time points so it could be much faster. Identity of phosphatase etc is still unknown.
7 CaM_bind_PDE1AC

Pathway No. 221
720
(uM^-1 s^-1)
5
(s^-1)
Kd(bf) = 0.0069(uM)-  Substrate:
 PDE1
 CaM-Ca4

 Products:
 CaM.PDE1
  For olf epi PDE1, affinity is 7 nM. Assume same for brain. Reaction should be pretty fast. Assume kb = 5/sec. Then kf = 5 / (0.007 * 6e5) = 1.2e-3

 
Result: 1 - 7 of 7 rows are displayed



Database compilation and code copyright (C) 2005, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.