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Result: 1 - 5 of 5 rows are displayed

Reaction List for pathway AC (Pathway Number 251) in Accession AMPAR_traff_model1 (Accession Number 60)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name
Pathway Name / 
Pathway No.
KfKbKdtauReagents
1 CaM-bind-AC1AC

Pathway No. 251
49.9997
(uM^-1 s^-1)
1
(s^-1)
Kd(bf) = 0.02(uM)-  Substrate:
 CaM-Ca4
 AC1

 Products:
 AC1-CaM
  Half-max at 20 nM CaM (Tang et al JBC 266:13 8595-8603 1991 kb/kf = 20 nM = 12000 #/cell so kf = kb/12000 = kb * 8.333e-5
2 dephosph-AC2AC

Pathway No. 251
0.1
(s^-1)
0
(s^-1)
--  Substrate:
 AC2*

 Products:
 AC2
  Random rate.
3 dephosph-PDEAC

Pathway No. 251
0.01
(s^-1)
0
(s^-1)
--  Substrate:
 cAMP-PDE*

 Products:
 cAMP-PDE
  The rates for this are poorly constrained. In adipocytes (probably a different PDE) the dephosphorylation is complete within 15 min, but there are no intermediate time points so it could be much faster. Identity of phosphatase etc is still unknown.
4 CaM_bind_PDE1AC

Pathway No. 251
719.982
(uM^-1 s^-1)
5
(s^-1)
Kd(bf) = 0.0069(uM)-  Substrate:
 PDE1
 CaM-Ca4

 Products:
 CaM.PDE1
  For olf epi PDE1, affinity is 7 nM. Assume same for brain. Reaction should be pretty fast. Assume kb = 5/sec. Then kf = 5 / (0.007 * 6e5) = 1.2e-3
5 cAMP_diffusionAC

Pathway No. 251
300
(s^-1)
5.4
(s^-1)
Not applicable**-  Substrate:
 cAMP

 Products:
 cAMP_in_dend
  Represents diffusion, from a volume of 9e-20 to 5e-18. Assuming neck dimensions of 0.1 x 0.1 microns, this works out to a diffusion const of about 270 um^2/sec, which is pretty conservative. It is what cAMP does in frog cilia.
** This is a trasport reation between compartments of different volumes. Therefore Kd is not applicable. Please Note Kf, Kb units are in number of molecules instead of concentration

 
Result: 1 - 5 of 5 rows are displayed



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