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Reaction List for pathway Ras (Pathway Number 367) in Accession Ajay_Bhalla_2007_Bistable (Accession Number 79)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name
Pathway Name / 
Pathway No.
KfKbKdtauReagents
1 dephosph-GEFRas

Pathway No. 367
1
(s^-1)
0
(s^-1)
--  Substrate:
 GEF*

 Products:
 inact-GEF
2 
  • Ras-intrinsic-GT
    Pase
  • Ras

    Pathway No. 367
    0.0001
    (s^-1)
    0
    (s^-1)
    --  Substrate:
     GTP-Ras

     Products:
     GDP-Ras
      This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
    3 dephosph-GAPRas

    Pathway No. 367
    0.1
    (s^-1)
    0
    (s^-1)
    --  Substrate:
     GAP*

     Products:
     GAP
      Assume a reasonably good rate for dephosphorylating it, 1/sec
    4 CaM-bind-GEFRas

    Pathway No. 367
    199.999
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.005(uM)-  Substrate:
     inact-GEF
     CaM-Ca4

     Products:
     CaM-GEF
      We have no numbers for this. It is probably between the two extremes represented by the CaMKII phosph states, and I have used guesses based on this. kf=1e-4 kb=1 The reaction is based on Farnsworth et al Nature 376 524-527 1995 28 Feb 2006: Increased affinity 36-fold to account for Ca input to MAPK cascade, possibly folding in other pathway inputs. 21 April 2006: Altered affinity to same level as pkm_mapk21.g model to prevent spontaneous turnon. Kf = 200, Kb = 1.

     
    Result: 1 - 4 of 4 rows are displayed



    Database compilation and code copyright (C) 2005, Upinder S. Bhalla and NCBS/TIFR
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