|
Enter a Search String | | Special character and space not allowed in the query term.
Search string should be at least 2 characters long. |
Molecule Parameter List for nNOS | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
| nNOS participated as | Molecule | Sum total of | Enzyme | Substrate of an enzyme | Product of an enzyme | Substrate in Reaction | Product in Reaction | | No. of occurrences | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | | NOS_Iwanaga | 11 | Pathway |
NOS | | This model is based on Iwanaga T et al. Biochemistry (1999) 38(50):16629-35.The model features the catalytic activity of neuronal Nitric Oxide Synthase expressed in Escherichia coli in a four step reaction mechanism. The rates found in the model are used directly from their paper and have been determined from experiments performed at 25 C. |
nNOS acting as a Molecule in NOS_Iwanaga Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | nNOS | NOS_Iwanaga
Accession No. : 11 | NOS
Pathway No. : 65 | 0.5 | 0.0016667 | No | | Neuronal Nitric Oxide Synthase. Found in the neurons, activity is dependent on CaM binding, in response to Ca levels. Molecular weight ~160 kDa. |
nNOS acting as a Substrate in a reaction in NOS_Iwanaga Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | Ca-CaMbind-nNOS | NOS_Iwanaga
Accession No. : 11 | NOS
Pathway No. : 65 | 3.25
(uM^-1 s^-1) | 0.05
(s^-1) | Kd(bf) = 0.0154(uM) | - | Substrate
CaM-Ca4
nNOS
Product
Ca-CaM_nNOS
| | Those binding CaM have a high Kd, including nNOS, ~ <=10 nM. Calcium mediated nNOS activity is required for NO synthesis. Rates from experiments... The binding of CaM to NOS has been demonstrated to act as the trigger necessary for electron transfer and catalytic activity.(Marletta, Biochemistry, 1997;36:12337-12345) |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details. |
|
