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Molecule Parameter List for Ca-CaM_nNOS | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | | Stuehr_NOS | 12 | Pathway |
NOS | | This is a model of neuronal Nitric Oxide Synthase expressed in Escherichia coli based on Santolini J. et al. J Biol Chem. (2001) 276(2):1233-43. The rates have been converted by 2x for every 10 degrees C. The original temperature was 10 degree C, cell temperature is assumed 30 degree C, so the conversion factor is 4. |
Ca-CaM_nNOS acting as a Molecule in Stuehr_NOS Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | Ca-CaM_nNOS | Stuehr_NOS
Accession No. : 12 | NOS
Pathway No. : 66 | 0 | 0.0016667 | No | | |
Ca-CaM_nNOS acting as a Substrate in a reaction in Stuehr_NOS Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | arg_bind_nNOS | Stuehr_NOS
Accession No. : 12 | NOS
Pathway No. : 66 | 0.5
(uM^-1 s^-1) | 81
(s^-1) | Kd(bf) = 162(uM) | - | Substrate
Ca-CaM_nNOS
L-Arg
Product
FeIII
| | From Stuehr et al., Biochem, 1999, 38(38):12446-12451. Rates used as it is from their results. |
Ca-CaM_nNOS acting as a Product in a reaction in Stuehr_NOS Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | Ca-CaMbind-nNOS | Stuehr_NOS
Accession No. : 12 | NOS
Pathway No. : 66 | 3.25
(uM^-1 s^-1) | 0.05
(s^-1) | Kd(bf) = 0.0154(uM) | - | Substrate
CaM-Ca4
nNOS
Product
Ca-CaM_nNOS
| | Those binding CaM have a high Kd, including nNOS, ~ <= 10 nM. The binding of CaM to NOS has been demonstrated to act as the trigger necessary for electron transfer and catalytic activity. (Marletta, Biochemistry, 1997;36;12337-12345) |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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