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Molecule Parameter List for AMP

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*AMP* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	2	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
cAMP_pathway	25	Network	Shared_Object_cAMP_pathway, PKA, AC, Gs
This is a model of the canonical cAMP signaling pathway: Ligand->Receptor->G-protein->Cyclase->cAMP->PKA. It also includes phosphodiesterases to balance out cAMP formation.Bhalla US Methods Enzymol. 2002;345:3-23

AMP acting as a Molecule in cAMP_pathway Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
AMP	cAMP_pathway Accession No. : 25	AC Pathway No. : 136	1000	1000	Yes
Assumed buffered to 1 mM. Value is irrelevant to simulation.

AMP acting as a Product of an Enzyme in cAMP_pathway Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	cAMP-PDE / PDE	cAMP_pathway Accession No. : 25	AC Pathway No. : 136	19.8413	10	4	explicit E-S complex	Substrate cAMP Product AMP
	Best rates are from Conti et al Biochem 34 7979-7987 1995. Though these are for the Sertoli cell form, it looks like they carry nicely into alternatively spliced brain form. See Sette et al JBC 269:28 18271-18274 Km ~2 uM, Vmax est ~ 10 umol/min/mg for pure form. Brain protein is 93 kD but this was 67. So k3 ~10, k2 ~40, k1 ~4.2e-6
2	cAMP-PDE* / PDE*	cAMP_pathway Accession No. : 25	AC Pathway No. : 136	19.8413	20	4	explicit E-S complex	Substrate cAMP Product AMP
	This form has about twice the activity of the unphosphorylated form. See Sette et al JBC 269:28 18271-18274 1994. We'll ignore cGMP effects for now.

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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