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Molecule Parameter List for Lac | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by  color. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Statistics | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lac participated as | Molecule | Sum total of | Enzyme | Substrate of an enzyme | Product of an enzyme | Substrate in Reaction | Product in Reaction |
| No. of occurrences | 1 | 0 | 0 | 0 | 1 | 3 | 0 |
Accession and Pathway Details |
| Accession Name | Accession No. | Accession Type | Pathway Link |
| Repressilator | 68 | Network | Lambda-cl_gene, Lac_gene, TetR_gene |
| This is a network model termed as Repressilator in which LacI inhibit transcription of tetR which in turn inhibit transcription of Lambda phage. This model simulates the result as per the Fig 1c of the paper : Elowitz MB, Leibler S., Nature. 2000 Jan 20;403(6767):335-8. | |||
Lac acting as a Molecule in Repressilator Network
| Name | Accession Name | Pathway Name | Initial Conc. (uM) | Volume (fL) | Buffered | |
| Lac | Repressilator Accession No. : 68 | Lac_gene Pathway No. : 296 | 0 | 3 | No | |
| Lac translated from lac m-RNA Derived from Figure 1(c) stochastic version Elowitz, M. et al. (2000) Nature 403:335-338 | ||||||
Lac acting as a Product of an Enzyme in Repressilator Network
| Enzyme Molecule / Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents |
| lac_mRNA / lac_translation | Repressilator Accession No. : 68 | Lac_gene Pathway No. : 296 | 0.000555595 | 0.167 | 4 | Classical Michaelis-Menten V = Etot.S.Kcat/Km+S | Substrate Amino_Acids Product Lac |
| Translation of the lac mRNA. Rates from Box 1 Elowitz, M. et al (2000) Nature 403: 335-338 | |||||||
Lac acting as a Substrate in a reaction in Repressilator Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | |
| 1 | Lac_degradation | Repressilator Accession No. : 68 | Lac_gene Pathway No. : 296 | 0.0167 (s^-1) | 0 (s^-1) | - | - | Substrate Lac Product Degraded_lac |
| This reaction denotes the degradation of Lac the half life of which is ten minutes. t1/2 = 0.693/k where k denotes kf Note: Rates had to scaled by approx 1.4 to get desired levels Elowitz, M. et al. (2000) Nature 403:335-338 | ||||||||
| 2 | inhibition_ reaction | Repressilator Accession No. : 68 | TetR_gene Pathway No. : 297 | 1000.01 (uM^-1 s^-1) | 224 (s^-1) | Kd(bf) = 0.224(uM) | - | Substrate Lac TetR_operator Product 1st_bound |
| It signifies the first binding of the repressor with the operator Kf = 1 /nM/sec Kb = 224 /sec Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 | ||||||||
| 3 | inhibition_ reaction[1] | Repressilator Accession No. : 68 | TetR_gene Pathway No. : 297 | 1000.01 (uM^-1 s^-1) | 9 (s^-1) | Kd(bf) = 0.009(uM) | - | Substrate Lac 1st_bound Product 2nd_bound |
| It signifies the second binding of the repressor with the operator Kf = 1 /nM/sec Kb = 9 /sec Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 | ||||||||