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Molecule Parameter List for TetR_operator

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*TetR_operator* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	1	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Repressilator	68	Network	Lambda-cl_gene, Lac_gene, TetR_gene
This is a network model termed as Repressilator in which LacI inhibit transcription of tetR which in turn inhibit transcription of Lambda phage. This model simulates the result as per the Fig 1c of the paper : Elowitz MB, Leibler S., Nature. 2000 Jan 20;403(6767):335-8.

TetR_operator acting as a Molecule in Repressilator Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
TetR_operator	Repressilator Accession No. : 68	TetR_gene Pathway No. : 297	0.0222	3	No
The TetR operator under normal conditions drives transcription of TetR Elowitz, M. et al. (2000) Nature 403:335-338

TetR_operator acting as an Enzyme in Repressilator Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
TetR_operator / TetR_basal_ transcription	Repressilator Accession No. : 68	TetR_gene Pathway No. : 297	0.0005556	0.5	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate Nucleotides Product tetR_mRNA
Basal transcription of the Tet R gene. Rates from Box 1 Elowitz, M. et al. (2000) Nature 403:335-338

TetR_operator acting as a Substrate in a reaction in Repressilator Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
TetR_operator_ inhibition_ reaction	Repressilator Accession No. : 68	TetR_gene Pathway No. : 297	1000.01 (uM^-1 s^-1)	224 (s^-1)	Kd(bf) = 0.224(uM)	-	Substrate Lac TetR_operator Product TetR_operator_ 1st_bound
It signifies the first binding of the repressor with the operator Kf = 1 /nM/sec Kb = 224 /sec Box 1 Elowitz, M. et al. (2000) Nature 403:335-338

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