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Molecule Parameter List for RCC1-RanGTP

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
RCC1-RanGTP participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000011

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
RanGTPase70Network
Shared_Object_RanGTPase Nucleus Cytoplasm 
This model represents a concentration gradient of RanGTP across the nuclear envelope. This gradient is generated by distribution of regulators of RanGTPase. We have taken a log linear plot of graphs generated by GENESIS and compared with the experimental graphs.

RCC1-RanGTP acting as a Molecule in  
RanGTPase Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
RCC1-RanGTPRanGTPase
Accession No. : 70
Nucleus
Pathway No. : 305
012000No
RCC1-GTP.Ran Ran-GTP complex bound to RCC1 Nuclear volume = 1.2 pL = 1.2e-15 m^3 Footnote for Table 2 Gorlich D et al (2003) EMBO J 22(5):1088-1100

RCC1-RanGTP acting as a Substrate in a reaction in  
RanGTPase Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
GTP.Ran_releaseRanGTPase
Accession No. : 70
Nucleus
Pathway No. : 305
55
(s^-1)
99.9994
(uM^-1 s^-1)
Kd(cb) = 1.8182(uM)-Substrate
RCC1-RanGTP

Product
GTP.Ran-nuclear
RCC1
Release of GTP.Ran from GTP.Ran-RCC1 complex Kf = 55 /sec Kb = 1.0e+08 /M/sec = 1.0e+02 /uM/sec Christian Klebe et al (1995) Biochemistry 34:12543-12552. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100

RCC1-RanGTP acting as a Product in a reaction in  
RanGTPase Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
GTP_bindingRanGTPase
Accession No. : 70
Nucleus
Pathway No. : 305
0.6
(uM^-1 s^-1)
19
(s^-1)
Kd(bf) = 31.6669(uM)-Substrate
GTP
RCC1-Ran

Product
RCC1-RanGTP
Binding of GTP to GDP free Ran-RCC1 complex Kf = 0.6e+06 /M/sec = 0.6 /uM/sec Kb = 19 /sec Christian Klebe et al (1995) Biochemistry 34:12543-12552. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100



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