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Molecule Parameter List for bRaf

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*bRaf* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	1	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
mRNA synthesis	94	Network	kinetics, compartment_1, compartment_2
The model consists of three major pathways: Calcium-calmodulin dependent protein kinase IV (CaMKIV), Mitogen-activated protein kinase (MAPK) and Protein Phosphatase 1 (PP1). Each of these converged on CREB activation. We also modeled further interactions with Transducer of regulated CREB activity 1 (TORC1) and the protein kinase A (PKA) pathway.

bRaf acting as a Molecule in mRNA synthesis Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
bRaf	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	0.2	1000	No

bRaf acting as a Product of an Enzyme in mRNA synthesis Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
Rap1GAP / RapGAP2	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	1	2	100	explicit E-S complex	Substrate bRaf_Rap1GTP Product Rap1GDP bRaf

bRaf acting as a Substrate in a reaction in mRNA synthesis Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	B_dash_Raf_ bind_Rap1GTP	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	60 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.0083(uM)	-	Substrate Rap1GTP bRaf Product bRaf_Rap1GTP

2	Ras_dash_act_ dash_braf	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	60 (uM^-1 s^-1)	0.5 (s^-1)	Kd(bf) = 0.0083(uM)	-	Substrate GTP_dash_Ras bRaf Product braf_dash_GTP_ dash_Ras

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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