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Enzyme List for pathway Ras (Pathway Number 333) in Accession Ajay_Bhalla_2004_PKM_MKP3_Tuning (Accession Number 77) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.Further ordering can be done according to header.   arrow indicates that ordering is done according to ascending or descending order. |
| Enzyme Molecule / Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents | ||
| 1 | GEF-Gprot-bg / GEF-bg_act-ras | Ras Pathway No. 333 | 0.505051 | 0.02 | 4 | explicit E-S complex | Substrate: GDP-Ras Product : GTP-Ras | |
| Kinetics based on the activation of Gq by the receptor complex in the Gq model (in turn based on the Mahama and Linderman model) k1 = 2e-5, k2 = 1e-10, k3 = 10 (I do not know why they even bother with k2). Lets put k1 at 2e-6 to get a reasonable equilibrium More specific values from, eg.g: Orita et al JBC 268(34) 25542-25546 from rasGRF and smgGDS: k1=3.3e-7; k2 = 0.08, k3 = 0.02 | ||||||||
| 2 | GEF* / GEF*-act-ras | Ras Pathway No. 333 | 0.505051 | 0.02 | 4 | explicit E-S complex | Substrate: GDP-Ras Product : GTP-Ras | |
| Kinetics same as GEF-bg-act-ras | ||||||||
| 3 | GAP / GAP-inact-ras | Ras Pathway No. 333 | 1.01039 | 10 | 4 | explicit E-S complex | Substrate: GTP-Ras Product : GDP-Ras | |
| From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting. | ||||||||
| 4 | CaM-GEF / CaM-GEF-act-ras | Ras Pathway No. 333 | 0.505051 | 0.02 | 4 | explicit E-S complex | Substrate: GDP-Ras Product : GTP-Ras | |
| Kinetics same as GEF-bg_act-ras | ||||||||