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Reaction List for pathway PLC_g (Pathway Number 215) in Accession MAPK_network_2003 (Accession Number 50) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.   indicates that ordering is done according to ascending or descending order.Keq is calculated only for first order reactions. Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules] |
| Reaction Name | Pathway Name / Pathway No. | Kf | Kb | Kd | tau | Reagents | |
| 1 | Ca_act_PLC_g | PLC_g Pathway No. 215 | 180 (uM^-1 s^-1) | 10 (s^-1) | Kd(bf) = 0.0556(uM) | - | Substrate: PLC_g Ca Products: Ca.PLC_g | Nice curves from Homma et al JBC 263:14 6592-6598 1988 Fig 5c. The activity falls above 10 uM, but that is too high to reach physiologically anyway, so we'll ignore the higher pts and match the lower ones only. Half-max at 1 uM. But Wahl et al JBC 267:15 10447-10456 1992 have half-max at 56 nM which is what I'll use. |
| 2 | Ca_act_PLC_g* | PLC_g Pathway No. 215 | 12 (uM^-1 s^-1) | 10 (s^-1) | Kd(bf) = 0.8333(uM) | - | Substrate: Ca PLC_G* Products: Ca.PLC_g* | Again, we refer to Homma et al and Wahl et al, for preference using Wahl. Half-Max of the phosph form is at 316 nM. Use kb of 10 as this is likely to be pretty fast. Did some curve comparisons, and instead of 316 nM giving a kf of 5.27e-5, we will use 8e-5 for kf. 16 Sep 97. As we are now phosphorylating the Ca-bound form, equils have shifted. kf should now be 2e-5 to match the curves. |
| 3 | dephosph_PLC_g | PLC_g Pathway No. 215 | 0.05 (s^-1) | 0 (s^-1) | - | - | Substrate: Ca.PLC_g* Products: Ca.PLC_g |