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Enzyme List for pathway PLC_g (Pathway Number 79) in Accession Synaptic_Network (Accession Number 16) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.Further ordering can be done according to header.   arrow indicates that ordering is done according to ascending or descending order. |
| Enzyme Molecule / Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents | ||
| 1 | Ca.PLC_g / PIP2_hydrolysis | PLC_g Pathway No. 79 | 97.2222 | 14 | 4 | Classical Michaelis-Menten V = Etot.S.Kcat/Km+S | Substrate: PIP2 Product : DAG IP3 | |
| Wahl et al JBC 267(15) 10447-10456 1992. Homma et al JBC 263:14 1988 pp 6592. These parms are the Ca-stimulated form. This is close to Wahl's figure 7, which I am using as reference. Also see Nakanishi et al Biochem J 256 453-459 1998, Nishibe et al Science 250 :1253-1256 This model uses a rather low PIP2 of 10 uM. | ||||||||
| 2 | Ca.PLC_g* / PIP2_hydrolysis | PLC_g Pathway No. 79 | 19.7917 | 57 | 4 | Classical Michaelis-Menten V = Etot.S.Kcat/Km+S | Substrate: PIP2 Product : DAG IP3 | |
| Mainly Homma et al JBC 263:14 1988 pp 6592. These parms are the Ca-stimulated form. Wahl et al JBC 267:15 10447-10456 1992 say that the tyrosine phosphorylated form has 7X higher affinity for substrate than control. The PIP2 levels in this model are rather low, at 10 uM. | ||||||||