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Enzyme List for pathway MAPK (Pathway Number 317) in Accession Ajay_Bhalla_2004_PKM_Tuning (Accession Number 76) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.Further ordering can be done according to header.   arrow indicates that ordering is done according to ascending or descending order. |
| Enzyme Molecule / Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents | ||
| 1 | Raf-GTP-Ras / Raf-GTP-Ras.2 | MAPK Pathway No. 317 | 0.159094 | 0.3 | 4 | explicit E-S complex | Substrate: MAPKK-ser Product : MAPKK* | |
| Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591). | ||||||||
| 2 | Raf-GTP-Ras / Raf-GTP-Ras.1 | MAPK Pathway No. 317 | 0.159094 | 0.3 | 4 | explicit E-S complex | Substrate: MAPKK Product : MAPKK-ser | |
| Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591). | ||||||||
| 3 | Raf*-GTP-Ras / Raf*-GTP-Ras.2 | MAPK Pathway No. 317 | 0.159094 | 0.3 | 4 | explicit E-S complex | Substrate: MAPKK-ser Product : MAPKK* | |
| Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6 | ||||||||
| 4 | Raf*-GTP-Ras / Raf*-GTP-Ras.1 | MAPK Pathway No. 317 | 0.159094 | 0.3 | 4 | explicit E-S complex | Substrate: MAPKK Product : MAPKK-ser | |
| Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6 | ||||||||
| 5 | MAPKK* / MAPKKtyr | MAPK Pathway No. 317 | 0.0462963 | 0.3 | 4 | explicit E-S complex | Substrate: MAPK Product : MAPK-tyr | |
| The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5 | ||||||||
| 6 | MAPKK* / MAPKKthr | MAPK Pathway No. 317 | 0.0462963 | 0.3 | 4 | explicit E-S complex | Substrate: MAPK-tyr Product : MAPK* | |
| Rate consts same as for MAPKKtyr. | ||||||||