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Reaction List for pathway CaM (Pathway Number 259) in Accession CaMKII_noPKA_model3 (Accession Number 62) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.   indicates that ordering is done according to ascending or descending order.Keq is calculated only for first order reactions. Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules] |
| Reaction Name | Pathway Name / Pathway No. | Kf | Kb | Kd | tau | Reagents | |
| 1 | CaM-TR2-bind-Ca | CaM Pathway No. 259 | 71.999 (uM^-2 s^-1) | 72 (s^-1) | Kd(af) = 1(uM) | - | Substrate: CaM Ca Ca Products: CaM-TR2-Ca2 | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !).... |
| 2 | -Ca | CaM Pathway No. 259 | 3.6 (uM^-1 s^-1) | 10 (s^-1) | Kd(bf) = 2.7778(uM) | - | Substrate: CaM-TR2-Ca2 Ca Products: CaM-Ca3 | K3 = 21.5, K4 = 2.8. Assuming that the K4 step happens first, we get kb/kf = 2.8 uM = 1.68e6 so kf =6e-6 assuming kb = 10 |
| 3 | PSD | CaM Pathway No. 259 | 72 (uM^-2 s^-1) | 72 (s^-1) | Kd(af) = 1(uM) | - | Substrate: CaM-PSD Ca-PSD Ca-PSD Products: CaM-TR2-Ca2-PSD | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !).... |
| 4 | -Ca-PSD | CaM Pathway No. 259 | 3.6 (uM^-1 s^-1) | 10 (s^-1) | Kd(bf) = 2.7778(uM) | - | Substrate: CaM-TR2-Ca2-PSD Ca-PSD Products: CaM-Ca3-PSD | K3 = 21.5, K4 = 2.8. Assuming that the K4 step happens first, we get kb/kf = 2.8 uM = 1.68e6 so kf =6e-6 assuming kb = 10 |
| 5 | PSD | CaM Pathway No. 259 | 0.465 (uM^-1 s^-1) | 10 (s^-1) | Kd(bf) = 21.5048(uM) | - | Substrate: CaM-Ca3-PSD Ca-PSD Products: CaM-Ca4-PSD | Use K3 = 21.5 uM here from Stemmer and Klee table 3. kb/kf =21.5 * 6e5 so kf = 7.75e-7, kb = 10 |