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Enzyme List for pathway MAPK_Asthagiri_no_fb (Pathway Number 192)

 Molecule Name/
Site Name 		Km (uM) kcat (1/s)Ratio
(k2/k3)		Enzyme TypeSubstrate Product
1 Enzyme Activity:
E1*_deactiv

Enzyme Molecule:
P1		0.060.15explicit E-S complexE1*
E1
  kp1+ =6*10^8 M-1 min-1, kp1- =30 min-1, kpcat,1 =6 min-1 form Asthagiri and Lauffenburger 2001.
2 Enzyme Activity:
E1_activ

Enzyme Molecule:
E0*		0.060.15explicit E-S complexE1
E1*
  k1+ =6*10^8 M-1 min-1, k1- =30 min-1, kcat,1 =6 min-1 and km caluculated to 0.06 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
3 Enzyme Activity:
E2*_deactiv

Enzyme Molecule:
P2		0.060.15explicit E-S complexE2*
E2
  kp2+ =6*10^8 M-1 min-1, kp2- = 30 min-1, kpcat,2 = 6 min-1 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
4 Enzyme Activity:
E2_activ

Enzyme Molecule:
E1*		0.060.15explicit E-S complexE2
E2*
  k2+ =6*10^8 M-1 min-1, k2- =30 min-1, kcat,2 =6 min-1 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
5 Enzyme Activity:
E3*_deactiv

Enzyme Molecule:
P3		0.060.15explicit E-S complexE3*
E3
  kp3+ =6*10^8 M-1 min-1, kp3- =30 min-1, kpcat,3 =6 min-1 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
6 Enzyme Activity:
E3_activ

Enzyme Molecule:
E2*		0.060.15explicit E-S complexE3
E3*
  k3+ =6*10^8 M-1 min-1, k3- =30 min-1, kcat,3 =6 min-1 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
7 Enzyme Activity:
E4*_deactiv

Enzyme Molecule:
P4		0.060.15explicit E-S complexE4*
E4
  kp4+ = 6*10^8 M-1 min-1, kp4- =30 min-1, kpcat,4 =6 min-1 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
8 Enzyme Activity:
E4_activ

Enzyme Molecule:
E3*		0.060.15explicit E-S complexE4
E4*
  k4+ = 6*10^8 M-1 min-1, k4- =30 min-1, kcat,4 =6 min-1 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
9 Enzyme Activity:
E5*_deactiv

Enzyme Molecule:
P5		0.060.15explicit E-S complexE5*
E5
  kp5+ = 6*10^8 M-1 min-1, kp5- = 30 min-1, kpcat,5 = 6 min-1 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
10 Enzyme Activity:
E5_activ

Enzyme Molecule:
E4*		0.060.15explicit E-S complexE5
E5*
  k5+ =6*10^8 M-1 min-1, k5- =30 min-1, kcat,5 =6 min-1 from Anand R. Asthagiri and Douglas A. Lauffenburger (2001 Mar-Apr) Biotechnol Prog. 17(2):227-39
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