| | Molecule Name/
Site Name | Km (uM) | kcat (1/s) | Ratio
(k2/k3) | Enzyme Type | Substrate | Product |
| 1 |
Enzyme Activity:
TetR_basal_
transcription
Enzyme Molecule:
TetR_operator | 0.0005556 | 0.5 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Nucleotides
| tetR_mRNA
|
| | Basal transcription of the Tet R gene. Rates from Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 |
| 2 |
Enzyme Activity:
TetR_
transcription
Enzyme Molecule:
TetR_operator_
1st_bound | 0.0005556 | 0.0005 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Nucleotides
| tetR_mRNA
|
| | Transcription of the TetR gene with only one of its promoter sites activated. Rates from Box 1 Elowitz, M. et al (2000) Nature 403:335-338 |
| 3 |
Enzyme Activity:
TetR_
transcription1
Enzyme Molecule:
TetR_operator_
2nd_bound | 0.0005556 | 0.0005 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Nucleotides
| tetR_mRNA
|
| | Transcription of the TetR gene with both its promoter sites activated. Rates from Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 |
| 4 |
Enzyme Activity:
tetR_
translation
Enzyme Molecule:
tetR_mRNA | 0.000555595 | 0.167 | 4 | Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S | Amino_Acids
| TetR
|
| | Translation of the tetR mRNA. Rates from Box 1 Elowitz, M. et al. (2000) Nature 403:335-338 |
