| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | AC | 0.015 | 1000 | No |
| | AC is present at rather low levels. Here we use 0.015 uM which is meant to lump various isoforms. None of the isoforms nor the other specific regulators are included here. Jacobowitz, PhD thesis. |
| 2 | AMP | 1000 | 1000 | Yes |
| | Assumed buffered to 1 mM. Value is irrelevant to simulation. |
| 3 | ATP | 5000 | 1000 | Yes |
| | ATP is present in all cells between 2 and 10 mM. See Lehninger. It is assumed buffered since the metabolic activity will take care of its levels. |
| 4 | cAMP-PDE | 0.5 | 1000 | No |
| | The levels of the PDE are not known at this time. However, enough kinetic info and info about steady-state levels of cAMP etc are around to make it possible to estimate this at about 0.5 uM. |
| 5 | cAMP-PDE* | 0 | 1000 | No |
| | This form has about 2X activity as plain PDE. See Sette et al JBC 269:28 18271-18274 1994. |
| 6 | Gs.AC | 0 | 1000 | No |
| | This is the active form of the cyclase. |
