| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | E2 | 0.0003 | 0.0016667 | No |
| | E2tot. concn is 0.0003 uM from Huang and Ferrell |
| 2 | INPUT(E1) | 0.1 | 0.0016667 | No |
| | E1tot concn. = 0.0003 uM from Mathematica code received from Dr. Ferrell through personal communication. |
| 3 | MAPK | 1.2 | 0.0016667 | No |
| | Concn. is 1.2 uM from Huang and Ferrell 1996 |
| 4 | MAPK-P | 0 | 0.0016667 | No |
| | This is the single phosphorylated form of MAPK from Huang and Ferrell 1996 |
| 5 | MAPK-PP | 0 | 0.0016667 | No |
| | This is the double phosphorylated form of MAPK from Huang and Ferrell 1996 |
| 6 | MAPKK | 1.2 | 0.0016667 | No |
| | Concn. is 1.2 uM from Huang and Ferrell 1996 |
| 7 | MAPKK-P | 0 | 0.0016667 | No |
| | This is the single phosphorylated form of MAPKK from Huang and Ferrell 1996 |
| 8 | MAPKK-PP | 0 | 0.0016667 | No |
| | This is the double phosphorylated form of MAPKK from Huang and Ferrell 1996 |
| 9 | MAPKKK | 0.003 | 0.0016667 | No |
| | Concn. of MAPKKKtot=0.003 uM from Huang and Ferrell 1996 |
| 10 | MAPKKK* | 0 | 0.0016667 | No |
| | This is the activated form of MAPKKK from Huang and Ferrell 1996 |
| 11 | MAPKKPase | 0.0003 | 0.0016667 | No |
| | Concn. of mekpasetot i.e.,MAPKKpasetot=0.003 uM from Huang and Ferrrell 1996 |
| 12 | MAPKKPase[1] | 0.0003 | 0.0016667 | No |
| | Concn. of mekpasetot i.e.,MAPKKpasetot=0.0003 uM from Huang and Ferrell, 1996 |
| 13 | MAPKPase | 0.12 | 0.0016667 | No |
| | Concn. of mapkpasetot=0.12 uM from Huang and Ferrell 1996 |
| 14 | MAPKPase[1] | 0.12 | 0.0016667 | No |
| | Concn. of mapkpasetot =0.12 uM from Huang and Ferrell 1996 |
