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Molecule List for pathway Shared_Object_Ajay_Bhalla_2007_PKM (Pathway Number 369)
| Name | Initial Conc. (uM) | Volume (fL) | Buffered | ||
| 1 | Ca | 0.08 | 1.5 | No | |
| 2 | Ca_input | 0.08 | 1.5 | Yes | |
| 3 | DAG | 12.1999 | 1.5 | Yes | |
| 4 | MAPK* | 0 | 1.5 | No | |
| 5 | MKP-1 | 0.02 | 1.5 | No | |
| MKP-1 dephosphoryates and inactivates MAPK in vivo: Sun et al Cell 75 487-493 1993. Levels of MKP-1 are regulated, and rise in 1 hour. Kinetics from Charles et al PNAS 90:5292-5296 1993. They refer to Charles et al Oncogene 7 187-190 who show that half-life of MKP1/3CH134 is 40 min. 80% deph of MAPK in 20 min Sep 17 1997: CoInit now 0.4x to 0.0032. See parm searches from jun96 on. | |||||
| 6 | PKC-active | 0 | 1.5 | No | |
| 7 | PPhosphatase2A | 1 | 1.5 | No | |
| Refs: Pato et al Biochem J 293:35-41(93); Takai&Mieskes Biochem J 275:233-239 k1=1.46e-4, k2=1000,k3=250. these use kcat values for calponin. Also, units of kcat may be in min! revert to Vmax base: k3=6, k2=25,k1=3.3e-6 or 6,6,1e-6 CoInit assumed 0.1 uM. See NOTES for MAPK_Ras50.g. CoInit now 0.08 Sep 17 1997: Raise CoInt 1.4x to 0.224, see parm searches from jun 96 on. | |||||
Summed Molecule List
| Target | Inputs | |
| 1 | PKC-active | PKC-Ca-memb* PKC-DAG-memb* PKM-zeta |
| Pathway Detail | Molecule List | Enzyme List | Reaction List |