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Reaction List for pathway NOS (Pathway Number 65)

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.
  Name KfKbKdtauSubstrateProduct
1 Ca-CaMbind-nNOS3.25
(uM^-1 s^-1)		0.05
(s^-1)		Kd(bf) = 0.0154(uM)-nNOS
CaM-Ca4
Ca-CaM_nNOS
  Those binding CaM have a high Kd, including nNOS, ~ <=10 nM. Calcium mediated nNOS activity is required for NO synthesis. Rates from experiments... The binding of CaM to NOS has been demonstrated to act as the trigger necessary for electron transfer and catalytic activity.(Marletta, Biochemistry, 1997;36:12337-12345)
2 k125
(s^-1)		0
(s^-1)		--nNOS.arg
nNOS.OH.Arg
  Rates from Iwanaga et al., Biochem, 1999;38(50):16629-16635. Obtained from rapid quenching expts at 25 C. >>>>>> 25 +/- 2.0
3 k24.4
(s^-1)		0
(s^-1)		--nNOS.OH.Arg
OH.Arg
  Rates from Biochem., 1999, 38(50), 16629-16635. obtained from rapid quenching expts at 25 C.... >>>>>> 4.4 +/- 0.5
4 k319
(s^-1)		0
(s^-1)		--nNOS.OH.Arg
nNOS.Cit+NO
  Rate from Biochem., 1999, 38(50), 16629-16635. Obtained from rapid queching expts at 25 C >>>>>> 19 +/- 3.0
5 k42.7
(s^-1)		0
(uM^-1 s^-1)		--nNOS.Cit+NO
Cit
NO
  Rate from Biochem., 1999, 38(50), 16629-16635., from rapid quenching expts at 25 C >>>>>> 2.7 +/- 0.3
6 nNOSbindArg0.5
(uM^-1 s^-1)		81
(s^-1)		Kd(bf) = 162(uM)-L-Arg
Ca-CaM_nNOS
nNOS.arg
  From Stuehr et al., Biochem,1999,38(38):12446-12451. Steuhr et al., from stopped flow expts of sub binding to nNOS report 0.5 (k-1) and 81 (k2). Rates used straight from results.
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