| |
Name | Accession
Type | Initial
Conc.
(uM) | Volume
(fL) | Buffered | Sum Total Of |
| 1 | AC1 | Network | 0.0741 | 0 | No | - |
| | Starting conc at 20 nM. Reduced conc to 10 nM, 14.8. Arnold 17 Feb 2005. Raised conc back to about 18 nM, for an nInit of precisely 1. 18 Feb. Doubled conc, to compensate for reduced ATP. 18 Feb. Doublec conc again, halved Vmax. |
| 2 | AC1-CaM | Network | 0 | 0 | No | - |
| | This version of cyclase is Calmodulin activated. Gs stims it but betagamma inhibits. |
| 3 | AC2 | Network | 0.0741 | 0 | No | - |
| | Starting at 0.015 uM. 17 Feb 2005: Raised slightly so as to have integer number of molecules. 18 Feb 2005: Doubled, to compensate for reduced ATP. |
| 4 | AC2* | Network | 0 | 0 | No | - |
| | This version is activated by Gs and by a betagamma and phosphorylation. |
| 5 | AMP | Network | 0 | 0 | No | - |
| 6 | ATP | Network | 2000 | 0 | Yes | - |
| | ATP is present in all cells between 2 and 10 mM. See Lehninger |
| 7 | CaM.PDE1 | Network | 0 | 0 | No | - |
| | Activity up 6x following Ca-CaM binding. |
| 8 | cAMP | Network | 0 | 0 | No | - |
| | The conc of this has been a problem. Schaecter and Benowitz use 50 uM, but Shinomura et al have < 5. So I have altered the cAMP-dependent rates in the PKA model to reflect this. |
| 9 | cAMP-PDE | Network | 0.5556 | 0 | No | - |
| | The levels of the PDE are not known at this time. However, enough kinetic info and info about steady-state levels of cAMP etc are around to make it possible to estimate this. 18 Feb 2005: After some playing with initial conc, it is now back at 0.5 uM. |
| 10 | cAMP-PDE* | Network | 0 | 0 | No | - |
| | This form has about 2X activity as plain PDE. See Sette et al JBC 269:28 18271-18274 1994. |
| 11 | cAMP_in_dend | Network | 0 | 0 | No | - |
| | Assume a 5 uM long segment of 1 uM^2 Xsection dendrite. |
| 12 | PDE1 | Network | 2.5926 | 0 | No | - |
| | CaM-Dependent PDE. Amount calculated from total rate in brain vs. specific rate. |
arrow indicates that ordering is done according to ascending or descending order.
and 
color.