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Enzyme List for pathway PLCbeta (Pathway Number 332) in Accession Ajay_Bhalla_2004_PKM_MKP3_Tuning (Accession Number 77) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.Further ordering can be done according to header.   arrow indicates that ordering is done according to ascending or descending order. |
| Enzyme Molecule / Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents | ||
| 1 | PLC-Ca / PLC-Ca | PLCbeta Pathway No. 332 | 19.8413 | 10 | 4 | explicit E-S complex | Substrate: PIP2 Product : DAG IP3 | |
| From Sternweis et al Phil Trans R Soc Lond 1992, also matched by Homma et al. k1 = 1.5e-5, now 4.2e-6 k2 = 70/sec; now 40/sec k3 = 17.5/sec; now 10/sec Note that the wording in Sternweis et al is ambiguous re the Km. | ||||||||
| 2 | PLC-Ca-Gq / PLCb-Ca-Gq | PLCbeta Pathway No. 332 | 5.00003 | 48 | 4 | explicit E-S complex | Substrate: PIP2 Product : DAG IP3 | |
| From Sternweis et al, Phil Trans R Soc Lond 1992, and the values from other refs eg Homma et al JBC 263(14) pp6592 1988 match. k1 = 5e-5/sec k2 = 240/sec; now 120/sec k3 = 60/sec; now 30/sec Note that the wording in Sternweis et al is ambiguous wr. to the Km for Gq vs non-Gq states of PLC. K1 is still a bit too low. Raise to 7e-5 9 Jun 1996: k1 was 0.0002, changed to 5e-5 | ||||||||