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Reaction List for pathway PLC_g (Pathway Number 79) in Accession Synaptic_Network (Accession Number 16)

Entries are grouped according to Pathway Number and they are alternately color coded using

and

color.
Further ordering can be done to the table header.

indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]

	Reaction Name	Pathway Name / Pathway No.	Kf	Kb	Kd	tau	Reagents
1	Ca_act_PLC_g	PLC_g Pathway No. 79	180 (uM^-1 s^-1)	10 (s^-1)	Kd(bf) = 0.0556(uM)	-	Substrate: PLC_g Ca Products: Ca.PLC_g
	Nice curves from Homma et al JBC 263:14 6592-6598 1988 Fig 5c. The activity falls above 10 uM, but that is too high to reach physiologically anyway, so we'll ignore the higher pts and match the lower ones only. Half-max at 1 uM. But Wahl et al JBC 267:15 10447-10456 1992 have half-max at 56 nM which is what I'll use.
2	Ca_act_PLC_g*	PLC_g Pathway No. 79	12 (uM^-1 s^-1)	10 (s^-1)	Kd(bf) = 0.8333(uM)	-	Substrate: Ca PLC_G* Products: Ca.PLC_g*
	Again, we refer to Homma et al and Wahl et al, for preference using Wahl et al JBC 267(15):10447-10456 1992. Half-Max of the phosph form is at 316 nM. Use kb of 10 as this is likely to be pretty fast. As we are phosphorylating the Ca-bound form, equils have shifted. kf should now be 2e-5 (Kf = 12) to match the reported half-max.
3	dephosph_PLC_g	PLC_g Pathway No. 79	0.05 (s^-1)	0 (s^-1)	-	-	Substrate: Ca.PLC_g* Products: Ca.PLC_g
	This is a generic balancing dephosphorylation step for the PLC. Rates are determined by considering balance between phosph and non-phosph forms of PLC-gamma. Wahl et al JBC 267(15) 10447-10456 1992 put the ratio at 50% phosph form in cytoplasm, about 20% in membrane.