| |
Reaction
Name | Pathway Name /
Pathway No. | Kf | Kb | Kd | tau | Reagents |
| 1 | Ca_act_PLC_g | PLC_g
Pathway No. 79 | 180
(uM^-1 s^-1) | 10
(s^-1) | Kd(bf) = 0.0556(uM) | - | Substrate:
PLC_g
Ca
Products:
Ca.PLC_g
|
| Nice curves from Homma et al JBC 263:14 6592-6598 1988 Fig 5c. The activity falls above 10 uM, but that is too high to reach physiologically anyway, so we'll ignore the higher pts and match the lower ones only. Half-max at 1 uM. But Wahl et al JBC 267:15 10447-10456 1992 have half-max at 56 nM which is what I'll use. | | 2 | Ca_act_PLC_g* | PLC_g
Pathway No. 79 | 12
(uM^-1 s^-1) | 10
(s^-1) | Kd(bf) = 0.8333(uM) | - | Substrate:
Ca
PLC_G*
Products:
Ca.PLC_g*
|
| Again, we refer to Homma et al and Wahl et al, for preference using Wahl et al JBC 267(15):10447-10456 1992. Half-Max of the phosph form is at 316 nM. Use kb of 10 as this is likely to be pretty fast. As we are phosphorylating the Ca-bound form, equils have shifted. kf should now be 2e-5 (Kf = 12) to match the reported half-max. | | 3 | dephosph_PLC_g | PLC_g
Pathway No. 79 | 0.05
(s^-1) | 0
(s^-1) | - | - | Substrate:
Ca.PLC_g*
Products:
Ca.PLC_g
|
| This is a generic balancing dephosphorylation step for the PLC. Rates are determined by considering balance between phosph and non-phosph forms of PLC-gamma. Wahl et al JBC 267(15) 10447-10456 1992 put the ratio at 50% phosph form in cytoplasm, about 20% in membrane. |
and 
color.
indicates that ordering is done according to ascending or descending order.