NCBS Home page
Accession List
Pathway List
Search
Authorized Users
Help
News archives

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By

Molecule Parameter List for CDK2cycA

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
CDK2cycA participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1100011

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • CDK2-CyclinA_
    activation_
    improved
    		• 43Pathway
    CDK2_CyclinA_activation_improved 
    This version of the model for CDK2/Cyclin A complex activation (expressed in E. coli) is a good match to the data and the simulation matches the graphs Fig.1B and Fig.1B inset. The rate constants though differ from the rate constants in Morris MC. et al. J Biol Chem. (2002) 277(26):23847-53.

    CDK2cycA acting as a Molecule in      CDK2-CyclinA_activation_improved Network
    NameAccession NamePathway NameInitial Conc.
    (uM)    		Volume
    (fL)    		Buffered
    CDK2cycA
  • CDK2-CyclinA_
    activation_
    improved
    Accession No. : 43
  • CDK2_CyclinA_
    activation_
    improved
    Pathway No. : 191
    		• 0999.98No
    CoInit = 0 uM Morris et al (2002 june 28) J Biol Chem. 277(26): 23847--23853
    Reaction scheme 1 pp.23848

    CDK2cycA acting as a Summed Molecule in      CDK2-CyclinA_activation_improved Network
    Accession NamePathway NameTargetInput
  • CDK2-CyclinA_
    activation_
    improved
    Accession No. : 43
  • CDK2_CyclinA_
    activation_
    improved
    Pathway No. : 191
    		• total_fluorescenceCDK2cycA
    CDK2cycA*
  • basal_
    fluorescence
    • The volume of this pool is scaled to 1/5.8 times the general volume in the model (1e-15 m^3) so that the range of fluorescent changes is 0.058 for complete consumption of the fluorophore (0.1 uM of Cdk2). The volume is therefore 0.1724e-15

    CDK2cycA acting as a Substrate in a reaction in      CDK2-CyclinA_activation_improved Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    Activation
  • CDK2-CyclinA_
    activation_
    improved
    Accession No. : 43
  • CDK2_CyclinA_
    activation_
    improved
    Pathway No. : 191
    		• 1.1
    (s^-1)    		0.6
    (s^-1)    		Keq = 0.5455(uM)0.588secSubstrate
    CDK2cycA

    Product
    CDK2cycA*
    Morris et al 2002 JBC 277(26): 23847--23853 kf and kb values not published kobs2 = 2.5+-0.1/sec kf = 1.1/sec and kb = 0.6/sec used in this simulation

    CDK2cycA acting as a Product in a reaction in      CDK2-CyclinA_activation_improved Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    Binding
  • CDK2-CyclinA_
    activation_
    improved
    Accession No. : 43
  • CDK2_CyclinA_
    activation_
    improved
    Pathway No. : 191
    		• 33
    (uM^-1 s^-1)    		50
    (s^-1)    		Kd(bf) = 1.5152(uM)-Substrate
    Cdk2
    CyclinA

    Product
    CDK2cycA
    kf = 33/sec/uM and kb = 50/sec differs from the values in Morris et al (2002 june 28) J Biol Chem. 277(26):23847-53
    Table 1 pp.23849 Published kf = 19/sec/uM and kb = 25/sec


    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
    This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.