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Name Notes Model

Molecule Parameter List for E2

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

E2 participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	1	1	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_Asthagiri_ enz_fb	46	Pathway	MAPK_Asthagiri_enz_fb
This is a complex model to examine mechanisms that govern MAPK pathway dynamics in Chinese hamster ovary (CHO) cell lines, particularly the role of enzyme targeted negative feedback mechanism in generating downstream signal adaptation. This model simulates the results as per the simulation conditions specified for figure 7B of the paper by Asthagiri AR and Lauffenburger DA. Biotechnol Prog. (2001) 17(2):227-39. However, there is some discrepancy with the outputs shown in Figure 7B.

E2 acting as a Molecule in  MAPK_Asthagiri_enz_fb Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
E2	MAPK_Asthagiri_ enz_fb Accession No. : 46	MAPK_Asthagiri_ enz_fb Pathway No. : 194	0.0427	390	No
E2^T =10^4 no./cell from Asthagiri and Lauffenburger 2001.

E2 acting as a Substrate for an Enzyme in  MAPK_Asthagiri_enz_fb Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
E1*  / E2_activ	MAPK_Asthagiri_ enz_fb Accession No. : 46	MAPK_Asthagiri_ enz_fb Pathway No. : 194	0.0600001	0.1	5	explicit E-S complex	Substrate E2 Product E2*
k2+ =6*10^8 M-1 min-1, k2- =30 min-1, kcat,2 =6 min-1 from Asthagire and Lauffenburger 2001

E2 acting as a Product of an Enzyme in  MAPK_Asthagiri_enz_fb Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
P2  / E2*_deactiv	MAPK_Asthagiri_ enz_fb Accession No. : 46	MAPK_Asthagiri_ enz_fb Pathway No. : 194	0.0600001	0.1	5	explicit E-S complex	Substrate E2* Product E2
kp2+ =6*10^8 M-1 min-1, kp2- = 30 min-1, kpcat,2 = 6 min-1 from Asthagiri and Lauffenburger 2001.