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Molecule Parameter List for TWA | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | | Chemotaxis | 57 | Pathway |
Chemotaxis | Reactions feeding into TnWa, TaWA and TWA are scaled by 10 Binding reaction of Tar with Aspartate has been scaled by 10 Phosphotransfer from CheAp to CheY has Kb = 0.263/sec/uM instead of 0.2/sec/uM used in BCT1.1.
All remaining parameters are from the .BCT files for BCT1.1 provided by Matthew Levin from the Computational Biology Group in the Department of Zoology at the University of Cambridge.
The June 2003 version of the BCT program is BCT4.3 and is available at the computational biology site of the Zoology department at Cambridge University.
Citation: Bray et al. Mol.Biol.Cell (1993) 4(5): 469-482. |
TWA acting as a Molecule in Chemotaxis Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | TWA | Chemotaxis
Accession No. : 57 | Chemotaxis
Pathway No. : 228 | 0.212 | 1.41 | No | | Tar-CheW-CheA complex TWA = 2.12e-07 M = 2.12e-1 uM As per Signal entry 13 in 1SIG_B.BCT provided by Matthew Levin Cell volume = 1.41e-15 L Table 1 pp.474 Bray et al 1993, Mol.Biol.Cell 4: 469-482 |
TWA acting as a Substrate in a reaction in Chemotaxis Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | Autophosphorylat
ion[1] | Chemotaxis
Accession No. : 57 | Chemotaxis
Pathway No. : 228 | 0.0757
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
CheA
TWA
Product
CheAp
| | TWA stimulated autophosphorylation of CheA TWA is complex of Tar, CheW and CheA Kf = 7.57*10e+04 /sec/M = 0.0757 /sec/uM As per Reaction 9 in 1REACT.BCT provided by Matthew Levin Kf = 5.9*10e+4 /sec/M = 0.059 /sec/uM Kb = 0 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 2 pp.475 Reaction Scheme 2 |
TWA acting as a Product in a reaction in Chemotaxis Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | Binding[2] | Chemotaxis
Accession No. : 57 | Chemotaxis
Pathway No. : 228 | 4
(uM^-1 s^-1) | 10
(s^-1) | Kd(bf) = 2.5(uM) | - | Substrate
CheA
TW
Product
TWA
| | | Binding of TW and CheA Kd = 0.3 uM and therefore Kf also suggested as 0.3 /sec/uM Kf = 4*10e5 /sec/M = 0.4 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 6 Rates used here are 10 times that stated, to allow for faster time courses seen during the drop of bias on removal of stimulus. | | 2 | Complexing[7] | Chemotaxis
Accession No. : 57 | Chemotaxis
Pathway No. : 228 | 4
(uM^-1 s^-1) | 10
(s^-1) | Kd(bf) = 2.5(uM) | - | Substrate
CheW
TA
Product
TWA
| | | TA and CheW complex formation Kf = 4*10e+05 /sec/M = 0.4 /sec/uM Kb = 1 /sec Table 3 pp.476 Reaction Scheme 7 Bray et al 1993, Mol.Biol.Cell 4: 469-482 Rates used here are 10 times that stated, to allow for faster time courses seen during the drop of bias on removal of stimulus. | | 3 | Binding[9] | Chemotaxis
Accession No. : 57 | Chemotaxis
Pathway No. : 228 | 4
(uM^-1 s^-1) | 10
(s^-1) | Kd(bf) = 2.5(uM) | - | Substrate
Tar
WA
Product
TWA
| | | Binding of Tar and CheW-CheA complex Kd = 2 uM and therefore Kf also suggested as 0.5 /sec/uM Kf = 4e+05 /sec/M = 0.4 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 8 Rates used here are 10 times that stated, to allow for faster time courses seen during the drop of bias on removal of stimulus. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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