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Molecule Parameter List for PLA2*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PLA2* participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000120

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2004_PKM_Tuning
    		• 76Network
    PKC,  Shared_Object_Ajay_Bhalla_2004_PKM_tuning,  PLA2,  
    PLCbeta,  Gq,  MAPK,  
    Ras,  EGFR,  Sos,  
    PLC_g,  CaMKII,  CaM,  
    PP1,  PP2B,  PKA,  
    AC,  PKM 
    This model is taken from the Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the reference feedforward model from Figure 8a.

    PLA2* acting as a Molecule in      Ajay_Bhalla_2004_PKM_Tuning Network
    NameAccession NamePathway NameInitial Conc.
    (uM)    		Volume
    (fL)    		Buffered
    PLA2*
  • Ajay_Bhalla_
    2004_PKM_Tuning
    Accession No. : 76
    		• PLA2
    Pathway No. : 314    		01.5No

    PLA2* acting as a Product of an Enzyme in      Ajay_Bhalla_2004_PKM_Tuning Network
    Enzyme Molecule /
    Enzyme Activity    		Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    MAPK*  /
    MAPK*
  • Ajay_Bhalla_
    2004_PKM_Tuning
    Accession No. : 76
  • Shared_Object_
    Ajay_Bhalla_
    2004_PKM_tuning
    Pathway No. : 312
    		• 25.6406104explicit E-S complexSubstrate
    PLA2-cytosolic

    Product
    PLA2*
    Km = 25uM @ 50 uM ATP and 1mg/ml MBP (huge XS of substrate) Vmax = 4124 pmol/min/ml at a conc of 125 pmol/ml of enz, so: k3 = .5/sec (rate limiting) k1 = (k2 + k3)/Km = (.5 + 0)/(25*6e5) = 2e-8 (#/cell)^-1 #s from Sanghera et al JBC 265 pp 52 , 1990. From Nemenoff et al JBC 268(3):1960-1964 - using Sanghera's 1e-4 ratio of MAPK to protein, we get k3 = 7/sec from 1000 pmol/min/mg fig 5

    PLA2* acting as a Substrate in a reaction in      Ajay_Bhalla_2004_PKM_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PLA2*-Ca-act
  • Ajay_Bhalla_
    2004_PKM_Tuning
    Accession No. : 76
    		• PLA2
    Pathway No. : 314    		6
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.0167(uM)-Substrate
    Ca
    PLA2*

    Product
    PLA2*-Ca
      To start off, same kinetics as the PLA2-Ca-act direct pathway. Oops ! Missed out the Ca input to this pathway first time round. Let's raise the forward rate about 3x to 5e-6. This will let us reduce the rather high rates we have used for the kenz on PLA2*-Ca. In fact, it may be that the rates are not that different, just that this pathway for getting the PLA2 to the memb is more efficien....
    2
  • dephosphorylate-
    PLA2*
  • Ajay_Bhalla_
    2004_PKM_Tuning
    Accession No. : 76
    		• PLA2
    Pathway No. : 314    		0.17
    (s^-1)    		0
    (s^-1)    		--Substrate
    PLA2*

    Product
    PLA2-cytosolic


    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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