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Molecule Parameter List for PLA2-Ca*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PLA2-Ca* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	2	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning	77	Network	Shared_Object_Ajay_Bhalla_2004_PKM_MKP3_Tuning,  PKC,  PLA2,   PLCbeta,  Ras,  Gq,   MAPK,  EGFR,  Sos,   PLC_g,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC,  MKP3,  PKM
This model is based on Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the feedforward model with MPK3 from figure 8a.

PLA2-Ca* acting as a Molecule in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PLA2-Ca*	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PLA2 Pathway No. : 331	0	1.5	No

PLA2-Ca* acting as an Enzyme in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PLA2-Ca* / kenz	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PLA2 Pathway No. : 331	20	5.4	4	explicit E-S complex	Substrate APC Product AA
10 x raise oct22 12 x oct 24, set k2 = 4 * k3

PLA2-Ca* acting as a Substrate in a reaction in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	PIP2-Ca-PLA2-act	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PLA2 Pathway No. : 331	0.012 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 8.3335(uM)	-	Substrate PLA2-Ca* temp-PIP2 Product PIP2-Ca-PLA2*
2	DAG-Ca-PLA2-act	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PLA2 Pathway No. : 331	0.003 (uM^-1 s^-1)	4 (s^-1)	Kd(bf) = 1333.3467(uM)	-	Substrate DAG PLA2-Ca* Product DAG-Ca-PLA2*
	27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.

PLA2-Ca* acting as a Product in a reaction in  Ajay_Bhalla_2004_PKM_MKP3_Tuning Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
PLA2-Ca-act	Ajay_Bhalla_ 2004_PKM_MKP3_ Tuning Accession No. : 77	PLA2 Pathway No. : 331	1 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate Ca PLA2-cytosolic Product PLA2-Ca*
Leslie and Channon BBA 1045 (1990) 261-270 fig6 pp267.